Associative chemosensing by fluorescent macrocycle-dye complexes – a versatile enzyme assay platform beyond indicator displacement.
نویسندگان
چکیده
A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.
منابع مشابه
Are two better than one? Comparing intermolecular and intramolecular indicator displacement assays in pyrophosphate sensors.
Peptide receptors with Zn(ii)-DPA units and a covalently bound fluorescent coumarin indicator on an oxazole-containing scaffold are shown to function as more selective pyrophosphate sensors than the analogous chemosensing ensembles in indicator displacement assays.
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An analytical method has been developed for the continuous monitoring of protease activity on unlabeled peptides in real time by fluorescence spectroscopy. The assay is enabled by a reporter pair comprising the macrocycle cucurbit[7]uril (CB7) and the fluorescent dye acridine orange (AO). CB7 functions by selectively recognizing N-terminal phenylalanine residues as they are produced during the ...
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A fluorogenic chemosensing system is described and shown to selectively detect pyrophosphate under physiological conditions. In the best case, pyrophosphate and hydrogen phosphate are capable of displacing a fluorescent coumarin-derived indicator from a bis Zn-dipicolylamine coordination compound with association constants of 10 and 10M , respectively. 2004 Elsevier Ltd. All rights reserved.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 24 شماره
صفحات -
تاریخ انتشار 2015